Staff Directory

Dr Daniel Scott

Laboratory Head, Receptor Structure and Drug Discovery Laboratory — Neuropeptides

I lead a group in the Neuropeptides division, focused on membrane protein engineering, structure and function. My work is focused on the development of new, generic protein engineering methods that can be applied to facilitate the application of structural biology and structure based drug design to traditionally challenging membrane protein targets such as G protein-coupled receptors (GPCRs). After completing my PhD on the relaxin and INSL3 receptors at The University of Melbourne in 2007, I worked as a postdoctoral researcher in the laboratory of Prof. Andreas Pl├╝ckthun in Zurich, Switzerland until the end of 2011. While in Zurich I invented a novel method, called CHESS, for the directed evolution of detergent-resistant membrane proteins. GPCRs stabilised with this method can be produced recombinantly in bacteria, purified and stored in detergents and experimentally probed as if they were highly stable soluble proteins. Such stabilised GPCRs can be applied to X-ray crystallography and NMR for structural studies, but can also be used to probe the molecular determinants of ligand binding, ligand-receptor selectivity and for drug discovery. I was recruited to The Florey in 2011 as a group leader, where my focus is on engineering and biophysical characterisation of GPCRs. In addition to my work on GPCRs, I enjoy developing new tools to help advance neuroscience (and other fields), such as denaturation resistant fluorescent proteins for cleared tissue imaging. I am a CI on four successful NHMRC project grants. I am also heavily involved in the commercialisation of research, being an inventor on 2 granted patents and a cofounder/shareholder of G7 Therapeutics AG. 

Research Papers

Listed below are Dr Daniel Scott's most recent publications.

To improve loading times, 1/2 pages of publications are being displayed Click here to load all of this researcher's publications.


N/A


Biodiesel synthesis assisted by ultrasonication using engineered thermo-stable Proteus vulgaris lipase

Journal

Fuel

Volume

208

Begin Page

430

Date

15/11/N/A

Author(s)

Shweta Gupta, Daniel Scott, Ratna Prabha, Muthupandian Ashokkumar
Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin

Journal

Scientific Reports

Volume

7

Begin Page

Date

12/6/N/A

Author(s)

Shoni Bruell, Ashish Sethi, Nicholas Smith, Daniel J Scott, Mohammed Akhter Hossain, Qing-Ping Wu, Zhan-Yun Guo, Emma J Petrie, Paul R Gooley, Ross AD Bathgate
Characterisation of a cell-free synthesised G-protein coupled receptor

Journal

Scientific Reports

Volume

7

Begin Page

Date

24/4/N/A

Author(s)

Patrick J Shilling, Fabian Bumbak, Daniel J Scott, Ross AD Bathgate, Paul R Gooley
Label-free screening of single biomolecules through resistive pulse sensing technology for precision medicine applications

Journal

Nanotechnology

Volume

26

Begin Page

Date

8/5/N/A

Author(s)

S Harrer, SC Kim, C Schieber, S Kannam, N Gunn, S Moore, D Scott, R Bathgate, S Skafidas, JM Wagner
The complex binding mode of the peptide hormone H2 relaxin to its receptor RXFP1

Journal

Nature Communications

Volume

7

Begin Page

Date

1/4/N/A

Author(s)

Ashish Sethi, Shoni Bruell, Nitin Patil, Mohammed Akhter Hossain, Daniel J Scott, Emma J Petrie, Ross AD Bathgate, Paul R Gooley
The relaxin peptide family and their novel G-protein coupled receptors

Journal

Letters In Peptide Science

Volume

10

Begin Page

393

Date

N/A

Author(s)

DJ SCOTT, TN WILKINSON, GW TREGEAR, R BATHGATE
Detection, localization, and action of the INSL3 receptor, LGR8, in rat kidney

Journal

Annals Of The New York Academy Of Sciences

Volume

1041

Begin Page

516

Date

N/A

Author(s)

P FU, P SHEN, C ZHAO, DJ SCOTT, C SAMUEL, JDC WADE, GW TREGEAR, R BATHGATE, AL GUNDLACH
Leucine-rich repeat-containing G-protein-coupled receptor 8 in mature glomeruli of developing and adult rat kidney and inhibition by insulin-like peptide-3 of glomerular cell proliferation.

Journal

Journal Of Endocrinology

Volume

189

Begin Page

397

Date

N/A

Author(s)

P Fu, PJ Shen, CX Zhao, DJ SCOTT, C SAMUEL, J WADE, G TREGEAR, R BATHGATE, A GUNDLACH
Modeling the Primary Hormone-Binding Site of RXFP1 and RXFP2

Journal

Annals Of The New York Academy Of Sciences

Volume

1160

Begin Page

74

Date

N/A

Author(s)

DJ SCOTT, G TREGEAR, R BATHGATE
Resolving the Unconventional Mechanisms Underlying RXFP1 and RXFP2 Receptor Function

Journal

Annals Of The New York Academy Of Sciences

Volume

1160

Begin Page

67

Date

N/A

Author(s)

BJ Hartley, DJ SCOTT, GE CALLANDER, TN Wilkinson, DE GANELLA, CKR KONG, S Layfield, T Ferraro, E PETRIE, R BATHGATE
Searching the human genome database for novel relaxin- and insulin-like peptides.

Journal

Letters In Peptide Science

Volume

8

Begin Page

129

Date

N/A

Author(s)

R BATHGATE, DJ SCOTT, SWL CHUNG, DW ELLYARD, AM GARREFFA, GW TREGEAR
Restricted expression of LGR8 in intralaminar thalamic nuclei of rat brain suggests a role in sensorimotor systems

Journal

Annals Of The New York Academy Of Sciences

Volume

1041

Begin Page

510

Date

N/A

Author(s)

P SHEN, P FU, KD PHELAN, DJ SCOTT, SL Layfield, GW TREGEAR, R BATHGATE, AL GUNDLACH
Characterization of the mouse and rat relaxin receptors

Journal

Annals Of The New York Academy Of Sciences

Volume

1041

Begin Page

8

Date

N/A

Author(s)

DJ SCOTT, SL Layfield, A RIESEWIJK, H MORITA, GW TREGEAR, R BATHGATE
Identification of the N-Linked Glycosylation Sites of the Human Relaxin Receptor and Effect of Glycosylation on Receptor Function

Journal

Biochemistry

Volume

47

Begin Page

6953

Date

N/A

Author(s)

Y YAN, DJ SCOTT, TN WILKINSON, Ji, G TREGEAR, R BATHGATE
Identification and characterization of the mouse and rat relaxin receptors as the novel orthologues of human leucine-rich repeat-containing G-protein-coupled receptor 7

Journal

Clinical And Experimental Pharmacology And Physiology

Volume

31

Begin Page

828

Date

N/A

Author(s)

DJ SCOTT, SL Layfield, A RIESEWIJK, H MORITA, GW TREGEAR, R BATHGATE
LGR7-truncate is a splice variant of the relaxin receptor LGR7 and is a relaxin antagonist in vitro

Journal

Annals Of The New York Academy Of Sciences

Volume

1041

Begin Page

22

Date

N/A

Author(s)

DJ SCOTT, GW TREGEAR, R BATHGATE
Defining the LGR8 Residues Involved in Binding Insulin-like Peptide 3

Journal

Molecular Endocrinology

Volume

21

Begin Page

1699

Date

N/A

Author(s)

DJ SCOTT, TN WILKINSON, SZ Zhang, TF Ferraro, J WADE, G TREGEAR, R BATHGATE
Characterization of the Rat INSL3 Receptor

Journal

Annals Of The New York Academy Of Sciences

Volume

1041

Begin Page

13

Date

N/A

Author(s)

DJ SCOTT, P FU, P SHEN, AL GUNDLACH, SL Layfield, A RIESEWIJK, H TOMIYAMA, JM HUTSON, GW TREGEAR, R BATHGATE
Solution structure and characterization of the receptor binding surface of insulin-like peptide 3*

Journal

Journal Of Biological Chemistry

Volume

281

Begin Page

28287

Date

N/A

Author(s)

K Rosengren, S Zhang, F Lin, N Daly, DJ Scott, R HUGHES, R Bathgate, D Craik, J Wade
Characterization of novel splice variants of LGR7 and LGR8 reveals that receptor signaling is mediated by their unique low density lipoprotein class A modules.

Journal

Journal Of Biological Chemistry

Volume

281

Begin Page

34942

Date

N/A

Author(s)

G TREGEAR, DJ SCOTT, S Layfield, Y YAN, S Sudo, AJW Hsueh, R BATHGATE
Characterization of the rat INSL3 receptor

Journal

Not Found

Volume

Not Found

Begin Page

34942

Date

N/A

Author(s)

D SCOTT, DJ Scott, P Fu, P Shen, A Gundlach, S Layfield, A Riesewijk, H Tomiyama, JM Hutson, GW Tregear, RAD Bathgate
The role of insulin 3, testosterone, mullerian inhibiting substance and relaxin in rat gubernacular growth.

Journal

Molecular Human Reproduction

Volume

8

Begin Page

900

Date

N/A

Author(s)

Y KUBOTA, C TEMELCOS, R BATHGATE, KJ SMITH, DJ SCOTT, CZ Zhao, JMH Hutson
Relaxin 3, a novel member of the insulin/relaxin superfamily of peptide hormones.

Journal

Biology Of Reproduction

Volume

66

Begin Page

249

Date

1/1/N/A

Author(s)

RA Bathgate, D Scott, CS Samuel, TCD Burazin, S Layfield, AA Claasz, NF Dawson, S Ma, C Bond, RJ Summers, JD Wade, GW Tregear
Modern perspectives on the structure, function and evolution of the relaxin-like peptides and their receptors

Journal

Current Medicinal Chemistry: Immunology, Endocrine And Metabolic Agents

Volume

5

Begin Page

369

Date

1/10/N/A

Author(s)

TN Wilkinson, D Scott, E Hopkins, RAD Bathgate
Ligand specificities of the relaxin (LGR7) and INSL3 (LGR8) receptors: Implications for relaxin and INSL3 action.

Journal

Biology Of Reproduction

Volume

68

Begin Page

313

Date

1/1/N/A

Author(s)

RAD Bathgate, D Scott, S Layfield, T Ferraro, JD Wade, GW Tregear

To improve loading times, 1/2 pages of publications are being displayed Click here to load all of this researcher's publications.