Quick Project Snapshot
High density multi-electrode array recording of in vitro networks in epilepsy
In this project the candidate will use high density extracellular multielectrode array recordings to investigate large scale network function. This a level of organization beyond that studied in Project 1 and will reveal fundamental properties of how the hippocampal and thalamocortical networks are altered in genetic models of epilepsy. The goal of these studies is to not only understand more about the neurobiology of epilepsy but also to create novel disease state models for creating anti-epileptic drugs. The method will involve cutting fresh brain slices and using 60 site multi-electrode arrays that enable electrical stimulation and recording from all sites simultaneously. Slices will be subject to various stimulation and pharmacological protocols to reveal aspects about excitability, synaptic transmission and plasticity.
Ion Channels and Human Diseases Laboratory
Our research focuses on understanding the pathology of ion channel disorders, in particular epilepsy, using in vitro and in vivo models to reveal opportunities for developing novel therapies. We use a multidisciplinary approach spanning ion channel biophysics, mouse transgenesis, genetic analysis, computational modeling and in vitro and in vivo electrophysiology.
All Projects by this Lab“CLARITY” based glass brain imaging in health and diseaseNeuroanatomical determinants of susceptibility in a model of genetic epilepsyMulti-site patch clamp recording of cortical micro networksProjects in network analysis of genetic epilepsyHigh content automated analysis of ion channels in epilepsyHigh density multi-electrode array recording of in vitro networks in epilepsyIn vivo electrophysiological analysis in mouse models of genetic epilepsyMRI tractography in mouse models of genetic epilepsy: Creation of prognostic and diagnostic structural biomarkersOptogenetic modulation of the area tempestas – an epilepsy hot spotTUBEROUS SCLEROSIS AND EPILEPSY: USING RESECTED TISSUE TO UNDERSTAND PATHOGENESIS
The Florey's Epilepsy division is a world-leading centre for epilepsy research. The division has major groups at both the Florey’s Austin and Parkville campus. The group studies mechanisms that cause epilepsy from the level of cells to the function of the whole brain. We use technologies including advanced MRI and cutting edge cellular physiology techniques to allow us to understand genetic and acquired mechanisms that give rise to epilepsy. Together with our colleagues from The University of Melbourne and across Australia we are working towards finding a cure for epilepsy.