Quick Project Snapshot

High content automated analysis of ion channels in epilepsy

Discovery of gene mutations in neurological disorders such as epilepsy is outstripping the ability to functionally validate them.  Because many epilepsy genes code for ion channels we have established high content automated patch clamp platforms based on the Nanion Patchliner 16 and the Fluxion HT 64 systems to bridge the "discovery" gap between genetics and functional validation.  Several new mutations have been found by our geneticist collaborators that are awaiting detailed functional analysis and the candidate will first have to produce mutant cDNAs then transiently transfect into HEK293 or CHO cells prior to analysis on the automated platforms.  Candidates will be trained in the necessary molecular biological methods and then in ion channel electrophysiology and will work closely with a senior member of the team to ensure success.

Milligan et al Annals of Neurology 2014

Epilepsy

The Florey's Epilepsy division is a world-leading centre for epilepsy research. The division has major groups at both the Florey’s Austin and Parkville campus. The group studies mechanisms that cause epilepsy from the level of cells to the function of the whole brain. We use technologies including advanced MRI and cutting edge cellular physiology techniques to allow us to understand genetic and acquired mechanisms that give rise to epilepsy. Together with our colleagues from The University of Melbourne and across Australia we are working towards finding a cure for epilepsy.

All Labs that operate in this Division

Epilepsy Cognition LaboratoryEpilepsy Neuroinformatics LaboratoryImaging and EpilepsyInnate Phagocytosis LaboratoryIon Channels and Human Diseases LaboratoryNeural Networks LaboratoryNeurophysiology of Excitable Networks LaboratoryPsychology and Experimental NeurophysiologySleep and CognitionTraumatic Brain Injury Laboratory