Quick Project Snapshot
Unravelling the role of chemokines in central control of the cardiovascular system
The brain controls the cardiovascular system via a complex network spanning a number of specialised brain structures. We are particularly interested in an area known as the paraventricular nucleus of the hypothalamus or PVN. While the projections from the PVN to other brain centres that control the heart and blood vessels are well documented, the neurotransmitters and signalling molecules utilised by these projections are less well known. In this project, we will determine the function of a new class of neuromodulators called chemokines. Chemokines are well known for their role in mounting an inflammatory response in the periphery but the recent discovery that chemokines might also act as a signalling molecule within the brain opens up an exciting avenue of research. This project will investigate the role of a specific chemokine known as CCL2 and its cognate receptor CCR2 in signalling to PVN neurones that project to another vitally important cardiovascular centre called the RVLM. This project will be relatively demanding and involve using a number of different techniques including electrophysiology if time permits. The successful completion of the project will increase our understanding of some of the fundamental mechanisms underpinning central cardiovascular regulation and control.
In this project we will be asking the following questions:
1. What is the distribution pattern of CCL2 and CCR2 within PVN and how many of these cells project to the RVLM.
2. How do PVN neurones react to exogenously applied CCL2?
small animal surgery (microinjection of neuronal tracers into the PVN)
immunohistochemistry (DAB and fluorescence)
microscopy (light, fluorescence, confocal)
in vitro electrophysiology (if time permits)
The Neuro-cardiovascular group is investigating the effects of the sympathetic nervous system in diseases including heart failure and septic shock.
All Projects by this LabUnravelling the role of chemokines in central control of the cardiovascular systemReinnervating the kidneysA novel therapy to maintain blood pressure and kidney function in septic shock
In Systems Neurophysiology we seek to learn how the nervous system controls various bodily functions and how that control is altered in disease. Our disease focus includes not only neurological disorders such as epilepsy and multiple sclerosis, but also how the nervous system impacts on non-neurological diseases such as heart failure and inflammatory diseases. A clear understanding of basic mechanisms is crucial in developing better therapies and reducing the impacts of illness.