Quick Project Snapshot
Multi-site patch clamp recording of cortical micro networks
In this project the candidate will be trained in the use of an emerging method in brain slice electrophysiology that allows for the simultaneous intracellular recording of 4 connected neurons. Using this recording mode it is possible to examine how neurons function in coupled micro networks in epileptic and normal brains to lead to a deeper understanding of the functional basis of epilepsy. If the candidate makes sufficient progress and is motivated this project may also expand into network analysis using multiphoton imaging where 50 or more neurons in a living brain can be labelled with a Ca2+ indicator dye and imaged in real time.
Ion Channels and Human Diseases Laboratory
Our research focuses on understanding the pathology of ion channel disorders, in particular epilepsy, using in vitro and in vivo models to reveal opportunities for developing novel therapies. We use a multidisciplinary approach spanning ion channel biophysics, mouse transgenesis, genetic analysis, computational modeling and in vitro and in vivo electrophysiology.
All Projects by this Lab“CLARITY” based glass brain imaging in health and diseaseNeuroanatomical determinants of susceptibility in a model of genetic epilepsyMulti-site patch clamp recording of cortical micro networksProjects in network analysis of genetic epilepsyHigh content automated analysis of ion channels in epilepsyHigh density multi-electrode array recording of in vitro networks in epilepsyIn vivo electrophysiological analysis in mouse models of genetic epilepsyMRI tractography in mouse models of genetic epilepsy: Creation of prognostic and diagnostic structural biomarkersOptogenetic modulation of the area tempestas – an epilepsy hot spotTUBEROUS SCLEROSIS AND EPILEPSY: USING RESECTED TISSUE TO UNDERSTAND PATHOGENESIS
The Florey's Epilepsy division is a world-leading centre for epilepsy research. The division has major groups at both the Florey’s Austin and Parkville campus. The group studies mechanisms that cause epilepsy from the level of cells to the function of the whole brain. We use technologies including advanced MRI and cutting edge cellular physiology techniques to allow us to understand genetic and acquired mechanisms that give rise to epilepsy. Together with our colleagues from The University of Melbourne and across Australia we are working towards finding a cure for epilepsy.