Quick Project Snapshot
“CLARITY” based glass brain imaging in health and disease
Recent improvements in the histochemical method of optically clearing whole tissues and the joint development of special optics that can image deep into them have created unprecedented views into the wiring of networks. Changes in wiring of cortical neurons have been implicated in a number of disorders such as epilepsy, schizophrenia and depression. In this project the candidate will prepare brains from mice with fluorescently labelled neurons and use 2 photon excitation or custom light sheet based microscopy to create 3D images in regions of the mouse cortex. By comparing normal and epilepsy models this work will begin to unravel the changes that occur prior to and after the occurrence of seizures. This will shed important light on the scale on which structural changes occur in epilepsy and will guide future experimental and clinical work.
Ion Channels and Human Diseases Laboratory
Our research focuses on understanding the pathology of ion channel disorders, in particular epilepsy, using in vitro and in vivo models to reveal opportunities for developing novel therapies. We use a multidisciplinary approach spanning ion channel biophysics, mouse transgenesis, genetic analysis, computational modeling and in vitro and in vivo electrophysiology.
All Projects by this Lab“CLARITY” based glass brain imaging in health and diseaseNeuroanatomical determinants of susceptibility in a model of genetic epilepsyMulti-site patch clamp recording of cortical micro networksProjects in network analysis of genetic epilepsyHigh content automated analysis of ion channels in epilepsyHigh density multi-electrode array recording of in vitro networks in epilepsyIn vivo electrophysiological analysis in mouse models of genetic epilepsyMRI tractography in mouse models of genetic epilepsy: Creation of prognostic and diagnostic structural biomarkersOptogenetic modulation of the area tempestas – an epilepsy hot spotTUBEROUS SCLEROSIS AND EPILEPSY: USING RESECTED TISSUE TO UNDERSTAND PATHOGENESIS
The Florey's Epilepsy division is a world-leading centre for epilepsy research. The division has major groups at both the Florey’s Austin and Parkville campus. The group studies mechanisms that cause epilepsy from the level of cells to the function of the whole brain. We use technologies including advanced MRI and cutting edge cellular physiology techniques to allow us to understand genetic and acquired mechanisms that give rise to epilepsy. Together with our colleagues from The University of Melbourne and across Australia we are working towards finding a cure for epilepsy.