Adam Walker
BSc (Hons) (Tas)
Brain Injury and Repair Group
Neurodegeneration Laboratory
Contact details
Email: adam.walker@florey.edu.au
Phone (office): +61 (0)3 8344 1869
Phone (lab): +61 (0)3 8344 5665
Fax: +61 (0)3 9348 1707
Supervisors
- Dr Julie Atkin
- Prof Malcolm Horne
PhD Project Title
- The role of the unfolded protein response in motor neuron disease.
Research Interests
- Currently researching molecular mechanisms of neurodegeneration, including non-classical secretion and endoplasmic reticulum stress responses, particularly in models of familial amyotrophic lateral sclerosis.
Laboratory Techniques
- Molecular biology
- vector design, construction, transformation, and purification
- site-directed mutagenesis
- DNA sequencing
- PCR, RT-PCR
- CHIP
- siRNA
- Cell culture
- primary neuronal and astrocyte culture
- continuous neuronal and astrocytic cell lines
- stable cell line generation
- transfection (liposome-mediated and electroporation)
- radioactive labelling
- toxicity and viability assays
- protein extraction
- Western blotting
- Immunocytochemistry
- ELISA
- Immunoprecipitation
Additional Information
- First year PhD student, Centre for Neuroscience
Students
