Dr Akhter Hossain
- Dr Akhter Hossain
BSc MSc (University of Dhaka), PhD (Tokyo Inst of technology)
Senior Research Officer
Neuropeptides Group
Peptide Laboratory
Contact Details
Email: | |
Phone: | +61 (0)3 8344 7330 (office) |
Mobile: | +61 (0)425 791 473 |
Research Interests
The primary goal of my research is to determine the molecular basis of the biological function of the protein, human relaxin, and to use the resulting information to prepare domain-minimised mimetics having either agonist or antagonist actions for use as potential therapeutics. There are two human relaxins known as relaxins-2 and -3. The former has clear roles in parturition and tissue remodelling. Relaxin-2 acts to regulate collagen synthesis and is proving to be very useful in reducing kidney and lung fibrosis. Additionally, over-expression of relaxin-2 has been associated with breast, prostate and thyroid cancers. In contrast, relaxin-3 is produced in the brain and is rapidly emerging as a key neuropeptide that seems to be involved in appetite and stress regulation. It has been shown to stimulate feeding and thus may have important therapeutic applications in cases of anorexia. Conversely an antagonist of the peptide may be a potential anti-obesity agent.
To date, I have exploited my considerable expertise in the solid phase chemical synthesis of these complex two chain, three disulfide bonded peptides to prepare numerous analogues of relaxins-2 and -3 for structure-function relationship mapping via NMR spectroscopic tertiary structural determination and receptor assay. My work has led to a clear elucidation of the active sites of the two peptides. The sites are structurally similar despite the two peptides acting on different receptors. I am now using the resulting information together with computer-aided drug design techniques to prepare domain-minimised analogues of the relaxins with therapeutically useful agonist or antagonist activities.
Laboratory Techniques
- Molecular Modelling, Solid phase synthesis and aqueous phase synthesis of linear and cyclic peptides (manual/automatic synthesis of peptides), their purification (HPLC/Gel filtration) and characterisation (MS and AA analysis)
- Circular dichroism (CD) spectroscopy, UV-vis spectroscopy, HPLC, MALDI TOF-MS and SELDI
Publications and Articles
Please see PubMed.
Mohammed Akhter Hossain, Keita Hamasaki, Hisakazu Mihara, and Akihiko Ueno* Chem. Lett. 2000 252-253.
Mohammed Akhter Hossain, Sachiko Matsumura, Takuya Toyoda, Keita Hamasaki, In: Szejtli J, ed. Cyclodextrins: From Basic Research to Market. Proceedings of the 10th International Cyclodextrin Symposium CD2000 Ann Arbour, Michigan, USA 2000 643-648.
Akihiko Ueno, Sachiko Matsumura, Mohammed Akhter Hossain, Hiroshi Tsutsumi, Takuya Toyoda, Tomoko Shimizu, Ryousuke Suzuki, Takuya Kanai, Keita Hamasaki, Hisakazu Mihara, and Akihiko Ueno* In: Szejtli J, ed. Cyclodextrins: From Basic Research to Market. Proceedings of the 10th International Cyclodextrin Symposium CD2000 Ann Arbour, Michigan, USA 2000 603-607.
Mohammed Akhter Hossain, Sachiko Matsumura, Takuya Kanai, Keita Hamasaki, Hisakazu Mihara, and Akihiko Ueno* J. Chem. Soc. Perkin Trans 2 2000 1527-1533.
Mohammed Akhter Hossain, Keita Hamasaki, Keiko Takahshi and Hisakazu Mihara, and Akihiko Ueno* J. Am. Chem. Soc. 2001 123 (30), 7435-7436.
Mohammed Akhter Hossain, Keiko Takahashi, Hisakazu Mihara, and Akihiko Ueno* J. Incl. Phen. 2002 43, 271-277.
Mohammed Akhter Hossain, Hisakazu Mihara*, and Akihiko Ueno J. Am. Chem. Soc. 2003 125 (37), 11178-11179.
Mohammed Akhter Hossain, Hisakazu Mihara*, and Akihiko Ueno Bioorg Med. Chem. Lett. 2004 24, 4305-4308.
Mohammed Akhter Hossain*, Soude Zhang, Feng Lin, Tania Ferraro, Ross A. D. Bathgate, Geoffrey W. Tregear, and John D. Wade Int. J. Peptide Res. Therap. 2006 12, 211-215.
Mohammed Akhter Hossain, Fabrice Thomas,* S. Hamman, E. Saint-Aman, Didier Boturyn, Pascal Dumy, and Jean-Louis Pierre J. Pept. Sci. 2006 9, 612-619.
Fazel Shabanpoor, Ross A. D. Bathgate, Mohammed Akhter Hossain, Eleni Giannakis, John D. Wade and Richard A. Hughes* J. Pept. Sci. 2007 13(2), 113-120
Samuel, C. S.; Lin, F.; Mohammed Akhter Hossain, Zhao, C.; Ferraro, T.; Bathgate, R. A. D.; Tregear, G. W.; Wade, J. D* Biochemistry 2007 46(18), 5374-5381.
Chester Kuei, Steven Sutton, Pascal Bonaventure, Cindy Pudiak, Jonathan Shelton, Jessica Zhu, Diane Nepomuceno, Jiejun Wu, Jingcai Chen, Fredrik Kamme, Mark Seierstad, Michael D. Hack, Ross A.D.Bathgate, Mohammed Akhter Hossain, John D. Wade, John Atack, Timothy W. Lovenberg, and Changlu Liu* J. Biol. Chem. 2007 282(35), 25425-254235.
Seong-Cheol Park, Mi-Hyun Kim, Mohammed Akhter Hossain, Song Yub Shin, Yangmee Kim, Lorenzo Stella, John D. Wade, Yoonkyung Park and Kyung-Soo Hahm* Biochim Biophys Acta 2008 1778, 229–241.
Mohammed Akhter Hossain, Ross A. D. Bathgate, Chze K. Kong, Fazel Shabanpoor, Suode Zhang, Linda M. Haugaard-Jönsson, K. Johan Rosengren, Geoffrey W. Tregear, and John D. Wade* ChemBioChem 2008 (in Press)
Mohammed Akhter Hossain, K. Johan Rosengren, Linda M. Haugaard-Jönsson, Soude Zhang, Sharon Layfield, Tania Ferraro, Norelle L. Daly, Geoffrey W. Tregear, John D. Wade and Ross A. D. Bathgate* J. Biol. Chem. 2008 (in press)
Abstracts of International Conferences:
Mohammed Akhter Hossain, Sachiko Matsumura, Takuya Kanai, Keita Hamasaki, Hisakazu Mihara, and Akihiko Ueno 6th International Symposium of the Society of Pure and Applied Coordination Chemistry (SPACC-6), The Hong Kong University of Science and Technology, Kowloon, Hong Kong, July 31-August 2, 1999.
Mohammed Akhter Hossain, Sachiko Matsumura, Takuya Kanai, Keita Hamasaki, Hisakazu Mihara, and Akihiko Ueno 10th International Cyclodextrin Symposium (CD-2000), University of Michigan, Ann Arbor, USA May 21st -May 24, 2000.
Mohammed Akhter Hossain, Keita Hamasaki, Keiko Takahashi, Hisakazu Mihara, and Akihiko Ueno 11th international symposium on Supramolecular Chemistry (ISSC-XI), Acros Fukuoka, Fukuoka, Japan, July 30-August 4, 2000
Mohammed Akhter Hossain, Keita Hamasaki, Keiko Takahashi, Hisakazu Mihara, and Akihiko Ueno Asian Cyclodextrin Conference 2002 (ACC-2002), Chiba, Japan.
Mohammed Akhter Hossain, Hisakazu Mihara*, and Akihiko Ueno 21st Cylodextrin Symposium 2003, Hokkaido, Japan.
Mohammed Akhter Hossain*, Soude Zhang, Feng Lin, Tania Ferraro, Ross A. D. Bathgate, Geoffrey W. Tregear, and John D. 3rd Intl Peptide Symposium in conjunction with 6th Australian Peptide Conference, Hamilton Island, Oct, 2005.
Mohammed Akhter Hossain*, Ross A. D. Bathgate, B. Van Lierop, A. J. Robinson, J. K. Rogengren and John D. Wade “Synthesis and bilogical activity of novel dicarba analogs of relaxin-3, an insulin like peptide (INSL7)” An official satellite symposium of 7th International Australian Peptide Symposium, Port Douglas, Oct, 2007.
Mohammed Akhter Hossain*, Ross A. D. Bathgate, J. W. Tregear, J. K. Rogengren and John D. Wade “Role of the A-chain on relaxin activity: An extensive structure-function study on human relaxins” An official satellite symposium of 7th International Australian Peptide Symposium, Port Douglas, Oct, 2007.
Patent
Bathgate RAD, Hossain MA, Samuel CS, Wade JD, Tregear GW "Modified relaxin polypeptides" US provisional patent application, filed 2007 (Application US 60/983768)
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